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DUTSCHER DOMINIQUE
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Image Search Results
Journal: Nature protocols
Article Title: Single-particle tracking photoactivated localization microscopy of membrane proteins in living plant tissues.
doi: 10.1038/s41596-020-00471-4
Figure Lengend Snippet: Fig. 8 | Mixture modeling reveals two latent subpopulations of mEos2-PHEVECT2 molecules. a, RStudio interface splitted in ‘Script’, ‘Console’ and ‘Environment/File/Plot/Packages/Help’ sections (Step 64). b, Histograms for Dinst and log10(Dinst) plots obtained for one cell expressing mEos2-PHEVECT2 and displayed for each cell in c (30 equally spaced bins by default). c, MM analysis from pooled six cells (‘hist_log10D_pool_suffix’ .tif output with ‘suffix’ type of the model). Top right, histogram of log10(Dinst) from pooled cells, with the components density of the best BIC MM plotted (GMM-2E here). Vertical black line indicates the optimal log10(Dinst) threshold and partitions mobile (orange) and immobile (blue) molecules. The dots indicate the estimated means of the MM. n corresponds to the number of molecules kept after filtering for the blinking and duration parameters. All the source data are available from http://bioserv.cbs.cnrs.fr/DOWNLOAD/sptPALM_data/.
Article Snippet: ● Jar & Rack staining jar, 50 slides (Dutscher, 048903), plastic rack (VWR, cat. no. 631-9520) ● Coverslips Menzel glaser 24 mm × 40 mm (VWR, MENZBB024040M113), 0.13–0.16-mm thickness ● Microscope slides (Sigma-Aldrich, cat. no. CLS294875X25) ● Corning Costar TC-Treated Multiple Well Plates, size 12 wells (Sigma-Aldrich, cat. no. CLS3513-50EA) ● Lens cleaning tissue (Thorlabs, cat. no. MC-5) ● Surgical tape, 12.5mm (VWR, cat. no. 115-8172) ● 0.22-μm filters (Merck, cat. no. SLGS033SS) ● Square Petri dishes (Dutscher, cat. no. 132511) ● Aluminum foil (Sigma-Aldrich, cat. no. Z185140) ● Razor blades (Dutscher, cat. no. 132511) ● Tweezers, Dumont #5 (Thermo Fisher Scientific, cat. no. 15483542) ● Microscope equipped with a TIRF arm and a TIRF objective (for example, Zeiss Elyra 7, Gataca Systems Ilas3 module and Leica DMi8 S module infinity TIRF) ● Ultrasensitive EMCCD camera dedicated to single-molecule detection (for example, Andor iXonEM+ or XU_897) ● Computer with at least 8 GB of RAM, an SSD disk with >100 GB and a multi-core processor Software ● ImageJ/FIJI (https://fiji.sc/) ● MATLAB runtime for R2019a, standalone set of shared libraries that enable the execution of compiled MATLAB applications or
Techniques: Expressing